词典目录
相关例句
1. Influence of A7-B7 disulfide bond Deletion on the Refolding and Structure of ProinsulinA7-B7二硫键缺失对胰岛素原重折叠及结构的影响(英文)
2. Site-directed Mutagenesis at disulfide bond Cys206-Cys210 of Prochymosin (Chymosin)
凝乳酶原(凝乳酶)二硫键Cys206-Cys210的定位突变
3. Hydrogen peroxide(H2O2) can reversible deactivate PTEN by forming a disulfide bond between the active -site of Cys124,Cys71.H2O2 can also control PTEN by Egr-1 in transcriptional level.
过氧化氢(H2O2)通过在活性位点半胱氨酸残基Cys124与Cys71之间形成二硫键,可逆地氧化灭活PTEN。 H2O2还可通过Egr-1在转录水平调控PTEN。
4. The Extracellular disulfide bond Cys20-Cys269 of CCR5 is Important for Its Conformational Integrity Maintenance and Function Performance
CCR5膜外二硫键Cys20-Cys269对其构象完整性以及功能行使起关键作用
5. Studies on the Function of disulfide bond Cys￣112-Cys￣115 in Arrowhead Proteinase Inhibitors
慈菇蛋白酶抑制剂中二硫键Cys1~（12）－Cys~（115）功能的研究
6. Five subunits(202 ku,171 ku,105 ku,80 ku 和73 ku) and one disulfide bond were detected.
卵中的卵黄蛋白总分子量为404 ku,有6个亚基(202 ku、188 ku、171 ku、105 ku、80 ku和66 ku);
7. It was found that PO of Octopus ocellatus is a protein with a molecular weight of 153.8 kDa, composed of two subunits with 75.6 kDa and 73.0 kDa, that probably linked by a disulfide bond.
PO分离纯化和生物化学性质鉴定结果发现,短蛸PO分子量可能为153.8 kDa,含有为75.6 kDa和73.0 kDa两个亚基。
8. SDS-PAGE analysis cofirmed that Momordica charantia seed proteins were mainly constituted by protein subunits 57KD and 49KD. They are combined by more LOW protein subunitd(47KD) through disulfide bond, and the proteins possess high medicine value and rich nutrient.
用SDS－PAGE分析表明：苦瓜种子主要含有57KD和49KD蛋白组分，它们由诸多低分子量（LMW＜47KD）蛋白亚基通过双硫桥结合，是药用价值高，营养丰富的蛋白资源。
9. The disulfide bond reducing agent dithiothreitol (DTT) significantly blocked As 2O 3 induced ΔΨm collapse and apoptosis.
巯基还原剂—二巯基苏糖醇（DTT）显著抑制As2O3诱导的ΔΨm下降。
10. Methods Protein with sulfhydryl or disulfide bond in 0.5 mol·L -1 NaOH, 1.5×10 -4 mol·L -1 Pb 2+ and 0.02% tetrabutylammonium iodide was heated in boiling water for 5 minutes. The reactive product gave a well defined reductive adsorption wave at -0.66 V (vs SCE) by means of single-sweep polarography, and the height of derivative wave was proportional to the concentration of proteins.
方法 含有巯基或二硫基的蛋白质与 0 5mol·L- 1 氢氧化钠、1 5× 10 - 4mol·L- 1 Pb2 + 和 0 0 2 %四丁基碘化铵的混合溶液在沸水浴中反应 5min ,采用单扫描极谱法 ,测定 - 0 6 6V(vsSCE)处一价导数波高。
11. ③ peptides with one disulfide bond;
③含有一个二硫键的多肽 ;
12. Synthesis of Novel disulfide bond-Bearing Cyclopeptides
新型含二硫键的环肽的合成
13. bond and Life
债券人生
14. Arbitrage in bond Market
国债市场中的套利操作
15. Preparation of Dicyclohexyl disulfide
二环己基二硫化物的合成
相关文摘
An alkaline phosphatase was partly purified from amphioxus by extraction, ammonium sulfate fractionation,pH regulation and get filtration with Sephadex G-100.Preliminary kinetic studies of the enzyme were undertaken. The results are as follows:The optimum pH of alkaline phosphatase for the hydrolysis of disodium phenyl phosphate is 10.1,and the optimum temperature is 40℃ .The Michaelis -Menten constant Km is1.13×10-3M at pH 10.1.Under given experimental conditions,the Michaelis-Menten constant Km varies with different pH values whereas the maximal velocity Vmax remains unchanged which indicates thatthe effect caused by pH is of the competitive type.The alkaline phosphate is strongly activated by Mg++ ion hut is inhibited by Zn++, EDTA,DFP,2-mercaptoethanol and PCMB. The Ki's of three inhibitors were determined and were found to be of the noncompetitive type. The marked inhibition of 2-mercaptoethanol on the enzyme suggests that the disulfide bond is easential to the maintainance of the enzyme activity. From the v versus pH plot,the pK value of the dissociation of the active group is 9.82 which suggests that -NH2 group might be an active group of the enzyme.The energy of activation is 3.16 kcal/mole, the temperature coefficient Q10(at 30-40℃)is 1.15.
从厦门文昌鱼中部份提纯一种碱性磷酸酶,并对该酶作用的动力学进行了初步的研究。该酶作用于底物磷酸苯二钠的最适pH为10.1,最适温度为40℃,测得其米氏常数值(K_m)为1.1×10~(-3)M,pH影响K_m值而不影响最大反应速度(V),表现为竞争性类型。Mg~(++)有明显的激活作用,而Zn~(++),EDTA,DFP,ME及PCMB等在一定浓度范围内,表现为非竞争抑制类型。ME的抑制作用表明硫硫键是维持酶活力所必需的。从pH对酶的活力影响,测得有关酶活性基团解离的pK值为9.82,在不同温度条件下,测得该酶的活化能为3.16仟卡/克分子,其温度系数为1.15(30—40℃)。
Three Toxic components, designated Aa-Toxin Ⅰ,Ⅱ,Ⅲ were isolated from the venom of Agkistrodon acutus by DEAE-sephadex A-50 chromatography and were further purified by gel filteration on Sephadex G-73, and by column chromotography on DE52 cellulose and Cm-sephaqex C-25. All these components show single band on acrylamide gel electrophoresis and one precipitin line on immuno-electrophoresis. They are distinct from each other immunochemically. Aa-Toxin Ⅰ and Aa-Toxin Ⅱ are acidic proteins, Aa-Toxin Ⅲ is basic. The Toxins demonstrated in our work have similar tool. wt about 22000. In addition to hemorrhagic and lethal activities, all these Toxins possess caseinolytic activities. The hemorrhagic and caseinolytic activities of these Toxins are inhibited by EDTA,cystein and the serum of homologous snake. disulfide bond and metals is probably involved in the stabilization of the conformation of these proteins. The amino acid composition of the Aa-Toxin Ⅰ was determined.
用DEAE-Sephadex A-50, Sephadex G-75, DE52纖維素和CM-SephadexC-25柱层析方法分离純化尖吻蝮蛇蛇毒,得到三种毒性組分,分别命名为五步蛇毒素Ⅰ、Ⅱ、Ⅲ。經聚丙烯醯胺凝胶电泳和琼脂免疫电泳鉴定均表現均一。毒素Ⅰ、Ⅱ为酸性蛋白,毒性Ⅲ为碱性蛋白,分子量均为22000左右,三者在免疫化学性质上是不同的。除出血和致死活性外,还都具有酪蛋白水解活性,它們的出血活性和酪蛋白水解活性可被EDTA,半胱氨酸和蛇血所抑制,金属和双硫键可能与其空間构象的稳定性有关。同时对毒素Ⅰ作了氨基酸組成分析。
The human hair keratin was examined by means of X-ray photoelectron spectro-scopy(XPS).The oxidation of disulfide to sulfonate is the major transformation whenthe hair is treated with hydrogen peroxide,but there have been much controversyin literatures about it.In addition,the oxidation of disulfide bonds at different positionson the surface of human hair has been revealed by XPS for the first time,approximately50% of the disulfide bonds in surface of long hair tip ends are oxidized duringweathering,and 20 to 30% of the disulfide bonds in surface of moderate long hair areoxidized during weathering.The mechanisms for oxidative degradation of disulfidesare discussed.A suggestion that this reaction is occurring largely by S-S fission,butcannot entirely rule out the possibility of C-S fission.
本文用电子能谱验证了头发角蛋白被H_2O_2等氧化剂氧化时二硫键的主要氧化产物是磺酸基团,从而回答了文献上有关这个问题长期存在的争论。此外文中首次用电子能谱揭示了长发不同部分的表面二硫键被自然氧化的不同情形,如揭示了长发末梢表面竟有50%左右的二硫键被自然氧化,中长发表面也有20～30%的二硫键被自然氧化。本文最后探讨了二硫键氧化降解机理,认为S-S 氧化断裂是主要的,C-S 断裂的可能性也不能完全排除。